10X ThermoPol II (Mg-free) Reaction Buffer and supplemental MgSO4 are optimized for use with VentR® and Deep VentR™ DNA Polymerases.
This buffer and supplement also provide superior reaction conditions for other thermophilic DNA polymerases, including Taq DNA Polymerase, as well as various other DNA and RNA modifying enzymes.
The Mg-free reaction buffer and supplemental MgSO4 are provided for users that require complete control over Mg2+ concentration to optimize their specific application.
See individual product descriptions for the recommended buffer for each enzyme, or visit the FAQ page for this product on the NEB web site for a complete list of enzymes for which this buffer is either recommended or compatible.