The BioLux Cypridina Luciferase Assay Kit contains the reagents necessary for assaying Cypridina Luciferase activity. Cypridina is a new secreted luciferase reporter from the ostracod Cypridina noctiluca. This luciferase does not require ATP and catalyzes the oxidation of its luciferin substrate in a photochemical reaction (1,2) (Figure 1). The substrate for Cypridina is different than coelenterazine, which is the common substrate of other marine luciferases including Renilla and Gaussia.