The NEBNext® Quick Ligation Module has been optimized to ligate efficiently DNA adaptors compatible with Illumina® sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with the NEBNext End Repair Module (NEB #E6050) and the NEBNext dA-Tailing Module (NEB #E6053) and is part of the original standard DNA library prep workflow, which is suitable for 1 – 5 μg of input DNA.