The NEBNext Fast DNA Library Prep Set for Ion Torrent is suitable when mechanical methods, such as Covaris® acoustic shearing, are preferred for fragmentation of input DNA. The components and protocol for this kit have been optimized for the volumes and concentrations of DNA sheared by mechanical methods, and the kit is compatible with input amounts from 10 ng – 1µg, with a workflow time of under 2 hours and ~12 min. hands-on time..