The NEB Golden Gate Assembly Mix contains an optimized mix of BsaI and T4 DNA Ligase. Together these enzymes, along with a highly optimized buffer, can direct the assembly of multiple inserts/modules using the Golden Gate approach. Also provided is the pGGA destination plasmid, which provides a backbone for your assembly, and features convenient restriction enzyme sites for subcloning, and has T7/SP6 promoter sequences to enable in vitro transcription.