FastLane Cell Multiplex Kits accelerate and streamline real-time RT-PCR and are ideal for experiments requiring rapid high-throughput gene expression analysis. The kits provide a set of buffers for preparing FastLane lysates from cultured cells. In addition to lysing cells, the buffers also stabilize cellular RNA and eliminate genomic DNA. FastLane lysates are used directly in multiplex, real-time one-step RT-PCR (with or without ROX passive reference dye). A variety of other detection formats are also available, including detection with SYBR Green or sequence-specific probes.
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