GeneRead qPCR SYBR Green Mastermixes contain all of the optimized reagents and buffers needed for SYBR Green real-time polymerase chain reactions in all real-time PCR instrumentation. Each master mix includes real-time PCR buffer, a high-performance HotStart DNA Taq polymerase, nucleotides, and a reference dye (ROX or fluorescein) if and when needed to normalize specific instruments’ optics. Simply add the master mix to PCR tubes along with template and primers. The chemically-modified and tightly controlled HotStart enzyme uniquely provides more accurate SYBR Green results by preventing the amplification of primer dimers and other nonspecific products.