This ready-to-use, optimized kit includes everything required for high-fidelity PCR — enzyme, buffers, and dNTPs. HotStar HiFidelity DNA Polymerase is a hot-start proofreading enzyme uniquely modified to produce A overhangs, enabling direct and streamlined UA/TA cloning. The buffer contains Factor SB to prevent degradation of primers and template during PCR setup, providing highly sensitive and reliable high-fidelity PCR. In addition, Q-Solution enables efficient amplification of “difficult” (e.g., GC rich) templates.