The miScript Microfluidics PCR Kit performs miRNA quantification on the Fluidigm BioMark System with samples that have been preamplified using the miScript Microfluidics PreAMP Kit. The miScript PCR System for microfluidics represents a breakthrough technology enabling accurate and comprehensive miRNA expression analysis on the Fluidigm BioMark System with as little as 10 ng total RNA. Highly multiplex, PCR-based preamplification of up to 400 miRNA-specific cDNA targets in one reaction results in a 1000–4000-fold amplification of each target. Preamplification dramatically reduces the sample requirements for analysis. A single 1 ng cDNA synthesis reaction can be used as template for up to 10 preamplification reactions.