The miScript Microfluidics PreAMP Kit performs preamplification to enable miRNA quantification on the Fluidigm BioMark System with a 96.96 or 48.48 Dynamic Array IFC. The miScript PCR System for microfluidics represents a breakthrough technology enabling accurate and comprehensive miRNA expression analysis on the Fluidigm BioMark System with as little as 10 ng total RNA. Highly multiplex, PCR-based preamplification of up to 400 miRNA-specific cDNA targets in one reaction results in a 1000–4000-fold amplification of each target. Preamplification dramatically reduces the sample requirements for analysis. A single 1 ng cDNA synthesis reaction can be used as template for up to 10 preamplification reactions.