The miScript Plant RT Kit is an unbiased, universal cDNA synthesis kit for small RNAs with 3’-end, 2’-O-Me modifications. The kit has been designed and optimized for real-time PCR-based small RNA expression analysis together with miScript SYBR Green PCR Kit and miScript miRNA PCR Arrays or miScript Primer Assays. These functionally verified assays and arrays are available for rice, corn, soybean, tobacco, and other significant species in agriculture and plant research. Small RNAs with a 2′-O-Me modification on the 3′ terminal base, like plant mature miRNAs and piwi-interacting RNAs (piRNAs), are refractory to polyadenylation, and cannot be efficiently reverse transcribed using a polyadenylation-based cDNA synthesis approach. The specialized miScript Plant RT Kit overcomes this by first ligating an adaptor to the 3′ terminus of plant miRNAs. Unlike polyadenylation, this ligation reaction is not negatively inhibited by the presence of a 2′-O-Me modification on the 3′ terminal base of plant miRNAs. Reverse transcription can be performed following ligation.