The QIAGEN One-Step Ahead RT-PCR Kit contains a blend of Sensiscript and Omniscript Reverse Transcriptases, a well-balanced combination of Taq DNA Polymerases and a proofreading enzyme. Heat-mediated activation of these enzymes enables reaction setup at room temperature. The easy one-tube setup and optimized components enable higher sensitivity and successful results. Additional tracing dyes allow the loading process to be monitored, minimizing pipetting errors.