The QIAGEN PCR Cloningplus Kit provides ready-to-use ligation reactions, which contain linearized cloning vectors that carry U overhangs at each 3′ end, allowing PCR products containing 3′-end A overhangs to be directly ligated and cloned with high efficiency and speed. The QIAGEN PCR Cloningplus Kit also provides competent E. coli cells and SOC medium for efficient transformation.