The QuantiNova Probe RT-PCR Kit enables sensitive quantification of RNA targets by real-time one-step PCR using sequence-specific probes. A combination of various integrated safety features removes variables and prevents artifacts, ensuring reliable gene expression profiling. The combination of a unique two-phase hot-start and PCR buffer system in the ready-to-use master mix allows room-temperature setup and ensures highly sensitive qRT-PCR on any real-time cycler. The QuantiNova Internal Control RNA can be used to test successful reverse transcription and amplification. It is intended to report instrument or chemistry failures, errors in assay setup and the presence of inhibitors. What’s more, the visual indicator reduces pipetting error while the integrated gDNA reduction step prevents overquantification of transcripts caused by genomic DNA carryover.