Limitations on the number and type of genomic analyses that can be performed due to insufficient quantities of genomic DNA can be overcome by global amplification of all DNA within a sample (whole genome amplification). The REPLI‑g Screening Kit provides DNA polymerase, buffers, and reagents for highly uniform whole genome amplification with minimal sequence bias and can be used with various starting materials, including genomic DNA, blood, and cultured cells. The streamlined, room-temperature procedure enables straightforward liquid handling that requires just 20 minutes. The kit is designed for rapid mutation screening using a range of genotyping, PCR, sequencing, or microarray assays. Amplified DNA can be used directly in most downstream applications without purification or quantification.