The Rotor-Gene SYBR Green PCR Demo Kit is for use in demonstrating the performance of the Rotor-Gene Q in combination with Rotor-Gene master mixes. The kit contains all the necessary reaction components to quantify a genomic DNA target by SYBR Green-based real-time PCR. These include prediluted genomic DNA standards, 2 unknown samples, an optimized master mix, and a primer pair specific for the human G protein-coupled estrogen receptor 1 (GPER) gene. Using the kit, the reliability, reproducibility, and sensitivity of gene quantification with the Rotor-Gene Q can be evaluated.