iQ Supermix is specially formulated for real-time PCR and has been tested for reliable amplification over a wide dynamic range of human genomic and plasmid DNA concentrations. It contains hot-start iTaq™ DNA Polymerase, dNTPs qualified for quantitative PCR, and buffer at concentrations optimal for real-time PCR assays. iQ Supermix facilitates reaction optimization and is qualified for use with all Bio-Rad real-time detection systems.