DNA Degradase Plus from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleoside components. Since nucleosides lack negatively charged phosphate, DNA Degradase Plus is ideal for whole-genome DNA methylation analysis by LC/MS. Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.
Quick and simple procedure for generating single nucleosides from DNA for quantitative analysis via LC/MS.
Convienent 2 hour, single enzyme digest v. conventional 6-16 hr multi-step enzyme digestion protocol