Our GpC Methylase completely methylates all cytosines within a 5′…GpC…3′ context in double-stranded DNA. The enzyme is specific for both non-methylated and hemimethylated DNA. The recombinant GpC Methylase is isolated from an E. coli strain that expresses the methyltransferase gene from Chlorella virus. The reaction conditions are optimized to maximize the processivity of the enzyme to ensure rapid, complete, and reproducible methylation of DNA for accurate DNA methylation analysis. This product is supplied with 10X Reaction Buffer and S-adenosylmethionine cofactor.
For complete, in vitro methylation of DNA for methylation analysis.
Methylation of chromatin DNA for DNA accessibility studies.
Inhibition of endonucleases with overlapping GpC sequence recognition.
[3H]-labeling of DNA.