The miRNeasy 96 Kit enables purification of total RNA, which includes RNA from approximately 18 nucleotides (nt) upwards from all types of animal tissues and cells, including difficult-to-lyse tissues. Alternatively, an miRNA-enriched fraction and a total RNA (>200 nt) fraction can be purified separately (for separate purification, an additional RNeasy 96 plate is required; for economical purchase of an additional RNeasy 96 plate and wash buffers, we recommend ordering an RNeasy 96 Kit). Manual purification is carried out using the Centrifuge 4K15C or the Centrifuge 4-16K and, optionally, the QIAvac 96 vacuum manifold.