The miRNeasy Mini Kit enables purification of total RNA, which includes RNA from approximately 18 nucleotides (nt) upwards from all types of animal tissues and cells, including difficult-to-lyse tissues. Alternatively, an miRNA-enriched fraction and a total RNA (>200 nt) fraction can be purified separately (for separate purification, an RNeasy MinElute Cleanup Kit is required). The miRNeasy Mini Kit is used for low-throughput RNA purification using spin columns. Copurification of miRNA and total RNA using the miRNeasy Mini Kit can be automated on the QIAcube.