The OneStep qMethyl Lite Kit provides a simple, straightforward, and bisulfite-free procedure for rapid, locus-specific DNA methylation assessment via the selective amplification of a methylated region of DNA. This is accomplished by splitting any DNA to be tested into two parts: a “Test Reaction” and a “Reference Reaction” (see figure below). DNA in the Test Reaction is digested with Methylation Sensitive Restriction Enzymes (MSREs) while DNA in the Reference Reaction is not. The “Lite” version allows real-time PCR to be performed with other fluorescent dyes or molecular probes of the researcher’s choosing.
Single step, bisulfite-free DNA methylation analysis.
Ideal for rapid screening of single and multi-locus DNA methylation
Omits the inclusion of SYTO 9® dye
Allows real-time PCR to be performed with other fluorescent dyes or molecular probes of the researcher’s choosing