pGLuc-Basic 2 is a cloning vector for expression in mammalian cells, containing a reporter gene but lacking promoter elements. The reporter gene is the secreted luciferase from the copepod Gaussia princeps. Gaussia Luciferase (GLuc) is a 19 kDa protein encoded by a “humanized” sequence, and it contains a native signal peptide at the N-terminus that allows it to be secreted from mammalian cells into the cell culture medium (1,2). The pGLuc-Basic 2 Vector contains a multiple cloning site (MCS) upstream of the GLuc coding sequence. A neomycin resistance gene under the control of an SV40 promoter allows selection for stable integration of the plasmid into the mammalian cell genome using G418.