The QuantiTect SYBR Green RT-PCR Kit provides highly specific quantification of RNA targets by real-time one-step RT-PCR using SYBR Green I detection. The combination of a hot start and a unique qRT-PCR buffer system ensures highly specific and sensitive real-time quantification of RNA targets. The dNTP mix includes dUTP, allowing optional treatment with UNG. The QuantiTect SYBR Green RT-PCR Kit is also supplied with an optimized RT mix for efficient and sensitive reverse transcription over a wide range of RNA template amounts. For convenience, the master mix can be stored at 2–8°C.