QuestTaq™ PreMix is supplied as a convenient 2X concentrated “master mix for robust PCR with little or no byproduct formation. It has been optimized for the non-biased amplification of cystosine, 5-methylcytosine (5-mC), 5-hydroxymethylcytosine (5-hmC), and glucosyl-5-hydroxymethylcytosine (g5-hmC) containing DNA, ensuring high yield amplification across a wide range of templates. The QuestTaq™ PreMix differs from QuestTaq™ qPCR PreMix in that it excludes SYTO® 9 dye from the PreMix solution, making it compatible with real-time and quantitative PCR with fluorescent dyes of the researcher’s choosing. QuestTaq™ DNA Polymerase has 3′-terminal transferase activity. The addition of “A” overhangs to amplified DNA makes it ideal for use in TA-cloning.
Premixed reagents for one-tube PCR.
Ideal for robust, non-biased amplification of 5mC, 5hmC, and g5hmC modified DNA.
Ideal for real-time, quantitative, and end-point analyses.
Compatible with a range of fluorescent dyes for use in real-time PCR