The RNeasy 96 Universal Tissue Kit enables total RNA purification in 96-well format from any tissue. Tissues are efficiently lysed and homogenized in QIAzol Lysis Reagent, and high-quality RNA is purified using silica-membrane RNeasy 96 plates. Manual purification is carried out using the QIAGEN 96-Well-Plate Centrifugation System and, optionally, the QIAvac 96 vacuum manifold. Automated purification using the related RNeasy 96 Universal Tissue 8000 Kit is performed on the BioRobot Universal System. Tissue samples can be conveniently stabilized using Allprotect Tissue Reagent, and efficiently disrupted using the TissueLyser II. Nonfatty tissues can be stabilized using the RNAlater RNA Stabilization Reagent.