The SMART-Seq HT Kit is designed to generate high-quality, full-length cDNA directly from 1–100 cells or 10 pg–1 ng of total RNA in a high-throughput manner. The kit is evolved from the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (SMART-Seq v4) and features a simplified protocol, which combines reverse transcription and PCR amplification into a convenient single step without compromising on performance.