– Input: 10 ng–3 µg of total RNA from spleen, lymph node, PBMCs, and hybridomas
– Sensitive and specific clonotype detection with optimized cDNA library generation
– Accurate amplification of mouse IgG subclasses and identification via sequencing in a majority of cases
– Optimized PCR pooling strategy for highly sensitive detection of different chains from the same sample
– Flexible pooling strategies accommodate different experimental requirements regarding alignment and identification of primary chain sequences