ZymoTaq™ DNA Polymerase contains all the reagents needed to perform “hot-start” PCR. The inclusion of a heat-activated, thermostable DNA polymerase reduces primer dimer and nonspecific product formation that can occur during PCR. This unique product is specifically designed for the amplification of bisulfite-treated DNA for methylation detection, but is applicable for conventional PCR. The product generates specific amplicons with little or no by-product formation. Simple and easy to use: Heat at 95°C for 10 minutes to initiate polymerization. ZymoTaq™ DNA Polymerase is a heat-activated, “hot start” polymerase that has 3′-terminal transferase activity. The addition of “A” overhangs to amplified DNA makes it ideal for use in TA-cloning.
Hot-start DNA polymerase for robust product formation.
Reduces non-specific PCR product formation from difficult templates (e.g., bisulfite converted DNA).
Compatible with real-time, quantitative PCR, and suitable for TA-cloning