Lung cancers are characterized by abundant genetic diversity, typically requiring the application of multiple assays in order to achieve cancer research insights. The GeneRead QIAact Lung DNA UMI combined with the GeneRead QIAact RNA Fusion UMI Panel to become the Lung All-in-One UMI Assay, is the first targeted gene panel to consolidate all relevant mutations into a single next-generation sequencing (NGS) assay.
The selection of the QIAact Lung DNA UMI Panel’s 550 variants across 19 genes, and the 79 fusions in genes commonly affected by translocations covered by the QIAact Lung RNA Fusion UMI Panel, were guided by the Lung Cancer Alliance; an international team of lung cancer research specialists, to ensure an assay of unparalleled relevance. Incorporating QIAGEN’s Unique Molecular Index (UMI) technology, these panels offer more accurate quantification and detection of molecular variants on the GeneReader than ever before, from as little as 40 ng of input DNA or 100 ng of total RNA.
To further streamline the library preparation protocol, the GeneRead QIAact Panel Cleanup Kit offers an optimized protocol automated on the QIAcube, saving time and preventing handling errors for DNA library preparation. Designed to simplify the workflow, these kit contain all regents required for both the target enrichment and library preparation steps of the GeneReader NGS System workflow.
*Currently liquid biopsy workflow is only available for GeneRead QIAact Lung DNA UMI Panel. Protocol for RNA extracted from liquid biopsy samples coming soon.
