The Ion 520™ & Ion 530™ ExT Kit-Chef enables robust, automated, and fast template preparation and sequencing of 400 to 600 base-read libraries using the Ion Chef™ System and the Ion S5™ and Ion S5™ XL Sequencing Systems. The Ion 520 & Ion 530 ExT Kit-Chef contains pre-packaged single-use template and sequencing reagent cartridges with integrated sample tracking, delivering an automated workflow for sequencing of 4 loaded chips with complete run traceability.