The miRNome is the most complete and accurate set of verified primers collected together in a single solution. Currently there are approximately thousands of miRNA that can be quantified using the QIAGEN miRNome. The content of miScript miRNA PCR Arrays is selected by updating our assay list to newly annotated miRNA found in miRBase, the database of miRNA, which ensures that each array is up-to-date and biologically relevant. These up-to-date miRNome miScript miRNA PCR Arrays are available for a variety of species including human, mouse, rat, dog and rhesus macaque.
Use of the Human miRNome miScript miRNA PCR Array maximizes the likelihood of discovering miRNA sequences whose expression patterns correlate with the biological phenotypes under study. A set of controls included on each plate enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. The Human miRNome miScript miRNA PCR Array enables easy and reliable, SYBR Green-based real-time PCR analysis of the expression of up to 2400 miRNA sequences and discovery of those most important to the research area of interest.