The miScript SYBR Green PCR Kit is an integral component of the miScript PCR System for miRNA detection and quantification. The kit is based on the proven technology of QuantiTect SYBR Green Kits. cDNA generated by the miScript II RT Kit is used as the template for real-time PCR with the miScript SYBR Green PCR Kit and either PCR arrays (miRNome or Pathway-Focused miScript miRNA PCR Arrays) or appropriate assays (miScript Primer Assays for miRNA or other noncoding RNA, miScript Precursor Assays for precursor miRNA, or QuantiTect Primer Assays for mRNA).