QIAseq UPX 3′ Transcriptome Kits enable high-throughput gene expression analysis from single cells and previously isolated RNA. Each kit is enough for 96 or 384 libraries, with kit format comprising of either a 96-well multi-break plates or 384-well single use plates with reverse transcription primers already lyophilized in the bottom of the plate for ease of use. To build libraries for RNA-seq, simply add 1 to 100 cells into each well or 10 pg to 1 ng of RNA to reconstitute a reverse transcription reaction. After cDNA synthesis, combine each plate into a single tube to complete the library preparation. All reagents necessary for starting with cells (such as lysis reagents) or RNA are included in the kit.