The Quick Dephosphorylation Kit contains Quick CIP, a heat-labile recombinant version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source. Quick CIP nonspecifically catalyzes the dephosphorylation of 5´ and 3´ ends of DNA and RNA phosphomonoesters. It also hydrolyses ribo- and deoxyribonucleoside triphosphates (NTPs and dNTPs). Quick CIP is useful in many applications that require the dephosphorylation of DNA or RNA ends. In cloning, dephosphorylation prevents re-ligation of linearized plasmid DNA. The enzyme can quickly dephosphorylate 5´ protruding, 5´ recessed, and blunt ends in just 10 minutes. Quick CIP may also be used to degrade unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis.